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Published: 2016-03-30
Abstract Views: 244
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DOI: https://doi.org/10.53747/jnst.v6i1.145
Issue
Vol. 6 No. 1 (2016)
Section
Articles
How to Cite
Nguyen, T. D., Doan, B., & Pham Thi , T. H. (2016). Immobilizing Bacillus subtilis on the carrier of poly (acrylic acid)/sodium bentonite for treating sludge from Pangasius fish ponds. Nuclear Science and Technology, 6(1), 43-49. https://doi.org/10.53747/jnst.v6i1.145

Immobilizing Bacillus subtilis on the carrier of poly (acrylic acid)/sodium bentonite for treating sludge from Pangasius fish ponds

Nguyen Thanh Duoc1, Doan Binh1, Pham Thi Thu Hong1
1 Research and Development Center for Radiation Technology, 202A Street 11, Linh Xuan ward, Thu Duc District, HCM. City

Main Article Content

Abstract

Sodium bentonite (NaBent) was modified by poly(acrylic acid) (PAAc) to prepare the carriers for immobilization of Bacillus subtilis. Different mixtures of NaBent/AAc were regularly dispersed in distilled water and irradiated under gamma rays at an absorbed dose of 6.5 kGy with dose rate of 0.85 kGy/hr in air for polymerization of acrylic acid and formation of poly(acrylic acid)/sodium bentonite (PAAc–NaBent). The reaction yield was determined with the initial concentration of acrylic acid (AAc). The functional group properties of the resulting PAAc–NaBent were analyzed by Fourier Transform Infrared spectra (FTIR). Bacillus subtilis cells were immobilized on both NaBent and PAAc–NaBent as carriers by adsorption method for treating the sludge contaminated by fish feces and residual feed from the Pangasius farming ponds. The results showed that immobilization capacity of Bacillus subtilis on the PAAc–NaBent was better than that on non-modified NaBent. Analysis of BOD for the farming pond water containing Bacillus subtilis and the bacteria immobilized carriers with time revealed the lower BOD values obtained with the samples containing PAAc–NaBent, suggested that degradation of organic pollutants by Bacillus subtilis immobilized on the PAAc–NaBent was faster than that by free bacteria.

Article Details

Keywords

Gamma irradiation, bentonite, acrylic acid, Bacillus subtilis

References

[1]. Clem A. G and Doehler R.W, “Industrial application of bentonite”, Clays and Clay minerals, Vol. 10, pp. 284-290, 1963.
[2]. L. J. Zhi, B. Han, T. Zhao and et. al, “Modification of montmorillonite through intercalative polymerization”, Chinese Chemical Letters, Vol. 14, No. 1, pp. 108-110, 2003.
[3]. K. G. Bhattacharyya, S. S. Gupta, “Kaolinite, montmorillonite, and their modified derivatives as adsorbents for removal of Cu(II) from aqueous solution”, Separation and Purification Technology, 50, pp. 388-397, 2006.
[4]. T. Wang, M. Wang, Z. Zhang, “Grafting of polymers from clay nanoparticles via high-dose gamma-ray irradiation”, Materials Letters, 61, pp. 3723-3727, 2007.
[5]. T. V. Son, T. V. Quy, “Research on Chromium removal in water by modified bentonite”, VNU Journal of Science, Volume 28, No. 1, pp. 37-43, 2012.
[6]. L. D. Trung, N. N. Linh and et. al, “Utilization of natural adsorbent materials for heavy metyl uptake from industrial waste sludge”, Science and Technology Development, Vol. 10, No. 1, pp. 63-70, 2007.
[7]. P. T. T. Hong and et. al, “ Study on grafting acrylic acid onto Montmorillonite by Gamm radiation to produce water absorbent hydrogel”, Proceeding of The 9th National Conference on Nuclear Science and Technology, pp. 859-864, 2011.
[8]. Comanescu, D. Ficai, I. Lacatusu, C. Guran, “New organic-inorganic hybrid materials for enzymes immobilization”, Rev. Chim. (Bucharest), Vol. 62, No. 1, pp. 37- 41, 2011.
[9]. M. Dogrua, R. Gul-Gven and S. Erdogan, “The use of Bacillus subtilis immobilized on Amberlite XAD-4 as a new biosorbent in trace metal determination”, Journal of Hazardous Materials, Vol. 149, pp. 166–173, 2007.
[10]. A. Poddar and S.C Jana, “Immobilization of hyperthermostable β-amylase from Bacillus subtilis DJ5 into gelatin film by glutaraldehyde crosslinking”, International Journal of Pharma and Bio Sciences, Vol. 2, pp. 77-86, 2011.
[11]. R. B. Mahto, Jagadeesh C. Bose K., “Production of alkaline protease from Bacillus subtilis by different entrapment techniques”, J Biochem Tech, pp. 498-501, 2012.
[12]. S. C. S. Martins, C. M. Martins, L. M. C. G. Fiúza and S. T. Santaella, “Immobilization of microbial cells: A promising tool for treatment of toxic pollutants in industrial wastewater”, African Journal of Biotechnology, Vol 12, No. 28, pp. 4412-4418, 2013.
[13]. G. Odian, Principles of Polymerization (4th Ed.), USA: John Wiley & Sons, Inc., pp. 729, 2004.
[14]. “Standard Methods for the Examintaion of Water and Wastewater 20th Edition”, American Public Heath Association.
[15]. S. Sieuwerts and et. al, “A simple and fast method for determining colony forming units”, Letters in Applied Microbiology, Vol. 47, pp. 275-278, 2008.
[16]. F. Kato and et. al, “Wastewater treatment with bacteria immobilized onto a ceramic carrier in an aerated system”, Journal Bioscience and Bioengineering, Vol. 95, No. 2, pp. 128-132, 2003.
[17]. T. T. M. Tam and N. T. Huong, “Optimization of Corynebacterium glutamicum immobilization process on bacterial cellulose carrier and its application for lysine fermentation”, IOSR Journal of Engineering, Vol. 7, pp. 33-38, 2014.
[18]. G.C. Delzer and S.W. McKenzie, “Five-Day Biochemical Oxygen Demand”, USGS TWRI Book (Third Edition), 2003.
[19]. P. Natkanski, A. Bialas and P. Kustrowski, “The synthesis of poly (acrylic acid)-bentonite and polyacrylamide-bentonite composites for adsorption applications”, Chemik, Vol. 66, No. 7, pp. 742-749, 2012.