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Published: 2012-03-30
Abstract Views: 49
DOI: https://doi.org/10.53747/nst.v2i1.276
Issue
Vol. 2 No. 1 (2012)
Section
Articles
How to Cite
LE , Q. L., NGUYEN , H. P. U., & VO , T. T. H. (2012). STUDY ON IN VITRO VARIATION GENERATION OF Cymbidium Labell "Anna Belle" BY γ-Co-60 IRRADIATION . Nuclear Science and Technology, 2(1), 51-58. https://doi.org/10.53747/nst.v2i1.276

STUDY ON IN VITRO VARIATION GENERATION OF Cymbidium Labell "Anna Belle" BY γ-Co-60 IRRADIATION

LE QUANG LUAN1, NGUYEN HUYNH PHUONG UYEN1, VO THI THU HA2
1 Center for Nuclear Techniques, Vietnam Atomic Energy Institute
2 Sai Gon Thuy Canh Corporation

Main Article Content

Abstract

Radiation ionization is one of the useful methods for mutation breeding of plants. In this study, γ-ray (Co-60) was used for generating morphological variations of Cymbidium Labell “Anna Belle”. The lethal doses of 50% samples (LD50) after 4 months cultivation were determined at 35.0Gy for protocorm like bodies (PLBs), 37.4Gy for in vitro shoot bubs and 70.1Gy for plantlets. PLBs were found as suitable samples for generation of variation of mentioned Cymbidium. The dose range from 20 to 50Gy was determined with a high ability of variation on C. Labell "Anna Belle". The highest frequency of variation was obtained about 3.82 x 10-3 at the dose of 30Gy. 157 in vitro variant plantlets were selected in M1V1 generation; among which , the variations were mainly found with 5 following types: removal of chlorophyll (8 plantlets), the leaves become shorter (64 plantlets), the leaves become longer (64 plantlets), increase of leaf number (11 plantlets) and the colour of pericardium was changed from green into violet (10 plantlets). Twenty one variant lines were selected in M1V2 generation.

Article Details

Keywords

Cymbidium, LD₅₀, Irradiation, Mutation, Protocorm like bodies, Variation

References

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