ANTIBACTERIAL ACTIVITY OF RADIATION DEGRADED CARBOXYMETHYL CHITOSAN

TRAN MINH QUYNH1, NGUYEN DUY LAM2, FUMIO YOSHII3
1 Hanoi Irradiation Center, Vietnam Atomic Energy Institute (VINATOM)
2 Vietnam Institute of Agricultural Engineering and Post-harvest Technology (VIAEP)
3 Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency (JAEA)

Main Article Content

Abstract

In this study, 10% aqueous solutions of carboxymethyl chitosan was irradiated at various doses using 60Co gamma source and radiation effects on its viscous average molecular weight were determined. In addition, carboxymethyl chitosan powder also irradiated with the same dose for comparison. Antimicrobial activities of carboxymethyl chitosan and its irradiated samples were investigated against Escherichia coli and Pseudomonas fruorences. The bacteria were cultured in the nutrient medium supplementing irradiated carboxymethyl chitosan, then optical density of culture fluid was measured using a turbidity. The results indicated antimicrobial activity of irradiated carboxymethyl chitosan not only depends on its concentration, but also depends on radiation dose, namely its molecular weight. The highest antimicrobial activity was observed for carboxymethyl chitosan irradiated at 100 kGy. This has been suggested that irradiation can be applied to improve the antimicrobial activity of carboxymethyl chitosan.

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References

[1] F. Shahidi., J. K. V. Arachchi., Y. J. Jeon, Food applications of chitin and chitosan, Trends in Food Science & Technology, 10, 37-51, 1999.
[2] N. D. Lam and T. M. Quynh, The use of chitosan as bioadhesive and its property improvement by irradiation for water-stable shrimp feed production, Journal of Chemistry, 40, 168-174, 2002.
[3] T. B. Diep., N. D. Lam., T. M. Quynh., T. Kume, Symposium Proceedings on Radiation Processing of Natural Polymers, JAERI-Conf., 2001-005, 17-27, 2002.
[4] L.Aral., Y. Kinumaki., Fujita T, Toxicity of chitosan, Bull. Tokai Reg. Fish. Res. Lab., 1968, 56-89.
[5] S. Matsuhashi and T. Kume, Enhancement of antimicrobial activity of chitosan by irradiation, J. Sci. Food Agric, 73, 237-241, 1997.
[6] N. D. Lam., T. B. Diep., T. M. Quynh., N. M. Hung., N. Nagasawa., T. Kume, Proc. Sym. on Radiation Application of Natural Polymer in Asia, JAERI-Conf., 2002-003, 117-130, 2003.
[7] X. F. Liu., Y. L. Guan., D. Z. Yang., Z. Li., K. D. Yao., Antibacterial Action of Chitosan and Carboxymethylated Chitosan, J. Appl. Polym. Sci., 79, 1324-1335, 2001.
[8] V. K. Mourya., N. N. Inamdar., A. Tiwari, Carboxymethyl chitosan and its applications. Adv. Mat. Lett. 1(1), 11-33, 2010.
[9] R. Jayakumar., M. Prabaharan., S. V. Nair., S. Tokura., H. Tamura., N. Selvamurugan, Novel carboxymethyl derivatives of chitin and chitosan materials and their biomedical applications, Prog. Mater. Sci., 56(7), 675-709, 2010.
[10] L. Zhao., H. Mitomo., N. Nagasawa., F. Yoshii., T. Kume, Radiation synthesis and characteristic of the hydrogels based on carboxymethylated chitin derivatives, Carbohyd. Polym., 51, 169-175, 2003.
[11] P. Wongpanit., N. Sanchavanakit., P. Pavasant., P. Supaphol., S. Tokura., R. Rujiravanit, Preparation and Characterization of Microwave-treated Carboxymethyl Chitin and Carboxymethyl Chitosan Films for Potential Use in Wound Care Application, Macromol. Biosci. 5, 1001-1012, 2005.
[12] W. Janvikul and B. Thavornyutikarn, New route to the preparation of carboxymethyl chitosan hydrogels, J. Appl. Polym. Sci., 90, 4016-4020, 2003.
[13] J. Ma., Y. Xu., Q. Zhang., L. Zha., B. Liang, Preparation and characterization of pH- and temperature-responsive semi-IPN hydrogels of carboxymethyl chitosan with poly (N-isopropyl acrylamide) crosslinked by clay, Colloid Polym Sci, 285, 479-484, 2007.
[14] Inoue Y., Kaneko M., Tokura S. Rep. Prog. Polym. Phys. in Japan 25, 759-760, 1982.
[15] M. J. Zohuriaan-Mehr, Advanced in chitin and chitosan modification through graft copolymerization: A comprehensive review, Iranian Polym. J., 14 (3), 235-265, 2005.
[16] G. D. Jiri, Radiation Technology for Polymers, CRS Press, 78-82, 2003.
[17] R. A. A. Muzzarelli., C. Muzzarelli., R. Tarci, M. Miliani., F. Garbbanelli., M. Cartolari, Biomacromol, 2, p. 165, 2002.