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Published: 2013-12-15
Abstract Views: 52
DOI: https://doi.org/10.53747/nst.v3i4.325
Issue
Vol. 3 No. 4 (2013)
Section
Articles
How to Cite
Dang , X. D., Bui , P. P., Tran , T. T., Le , A. Q., Dang , V. P., & Nguyen , Q. H. (2013). Study on gamma-irradiation degradation of chitosan swollen in H₂O₂ solution and its antimicrobial activityfor E. coli. Nuclear Science and Technology, 3(4), 32-39. https://doi.org/10.53747/nst.v3i4.325

Study on gamma-irradiation degradation of chitosan swollen in H₂O₂ solution and its antimicrobial activityfor E. coli

Dang Xuan Du1, Bui Phuoc Phuc2, Tran Thi Thuy3, Le Anh Quoc4, Dang Van Phu4, Nguyen Quoc Hien4
1 Hue University of Sciences, 77 Nguyen Hue street, Hue city
2 University of Natural Sciences Ho Chi Minh city, 227Nguyen Van Cu street, HCM. City
3 Nuclear Research Institute, 01 Nguyen Tu Luc street, Da Lat
4 Research and Development Center for Radiation Technology, 202A Street 11, Linh Xuan ward, Thu Duc District, HCM. City

Main Article Content

Abstract

Degradation of chitosan in swollen state with hydrogen peroxide solution (5% w/v) by γ-
irradiation was investigated. Molecular weight (Mw) of irradiated chitosan was determined by gel permeation chromatography (GPC). Fourier transform infrared (FT-IR) and ultraviolet-visible (UVvis) spectrawere analyzed to study the structure changes of degraded chitosan. The results showed that the chitosan of low Mw ~30-45 kDawas efficiently prepared by γ-irradiation of chitosan swollen in hydrogen peroxide solution at low dose less than 20kGy. The main structure as well as the degree of deacetylation of the degraded chitosan was almost no significant change. Furthermore, the radiation
degradation yield (Gs) was remarkably enhanced by the presence of H2O2. The obtained low Mw chitosan revealed high antimicrobial activity for E. colithat can be used for food preservation and other purposes as well.

Article Details

Keywords

Chitosan, degradation, E. coli, gamma-irradiation, hydrogen peroxide

References

[1]. W. Xia et al., “Biological activities of chitosan and chitooligosaccharides”,Food Hydrocolloids, 2011, 25:170–179.
[2]. NN. Duy et al., “Synergistic degradation to prepare oligochitosan by γ-irradiation of chitosan solution in the presence of hydrogen peroxide”,Radiation Physics and Chemistry, 2011, 80:848–853.
[3]. NQ. Hien et al., “Study on degradation to prepare oligochitosan by irradiation”, Vietnam Journal of Chemistry, 2000, 38:22–24 .
[4]. NQ. Hien et al., “Degradation of α-chitosan by conbined treatment with hydroperoxide and gamma 60Co radiation”, Nuclear Science and Technology, 2008, 4:44–59.
[5]. B.Kang et al., “Synergetic degradation of chitosan with gamma radiation and hydrogen peroxide”,Polymer Degradation and Stability, 2007, 92:359–362.
[6]. NM. El-Sawy et al., “Radiation-induceddegradation of chitosan for possible use as a growth promoter in agriculture purposes”,Carbohydrate Polymers, 2010, 79:555–562.
[7]. FCK. Ocloo et al., “Physicochemical and functional characteristics of radiation –
processed shrimp chitosan”,Radiation Physics and Chemistry, 2011, 80:837–841.
[8]. JZ. Knaul et al., “Characterization of deacetylated chitosan and chitosan molecular weight review”,Canadian Journal of Chemistry, 1998, 76:1699–1706.
[9]. J. Brugnerottoet al., “An infrared investigation in relation with chitin and chitosan characterization”,Polymer, 2001, 42: 3569– 3580.
[10]. JM. Wasikiewicz et al., “Green” molecular weight degradation of chitosan using microwave irradiation”,Polymer Degradation and Stability,2013, 98:863–867.
[11]. C. Qin et al., “Water-solubility of chitosan and its antimicrobial activity”, Carbohydrate Polymers, 2006, 63:367–374.
[12]. LY. Zheng, JF Zhu, “Study on antimicrobial activity of chitosan with different molecular weights”, Carbohydrate Polymers, 2003, 54:527–530.
[13]. RT. Woodsand AK.Pikaev (Ed.), Applied radiation chemistry:radiation processing, New York: John Wiley& Sons, 1994, 341–342.
[14]. P. Ulanskiet al., “OH – radical – induced chain scission of chitosan in absence and presence of dioxygen”. Journal of the Chemical Society Perkin Transaction, 2000, 2:2022–2028.
[15]. A. Charlesby, Atomic radiation and polymers, Oxford: Pergamon Press, 1960.
[16]. R.Czechowska-Biskup et al., “Degradation of chitosan and starch by 360-kHz ultrasound”, Carbohydrate Polymers, 2005, 60:175–184.
[17]. C.Peniche et al., “Self-curing membranes of chitosan/PPA IPNs obtained by radical polymerization: preparation characterization and interpolymercomplexation”,
Biomaterials,1999, 20:1869–1878.
[18]. A. Einbu et al., “Depolymerization and de-Nacetylation of chitin oligomers in hydrochloric acid”,Biomacromolecules, 2007, 8:309–314.
[19]. XF. Liu et al., “Antibacterial action of chitosan and carboxymethylated chitosan”, Journal of Applied Polymer Science, 2001, 79:1324–1335.
[20]. N. Liu et al., “Effect of MW and concentration of chitosan on antimicrobial activity of Escherichia coli”, Carbohydrate Polymers, 2006, 64:60–65.
[21]. CS. Lee et al., “Antibacterial activity of low molecular weight chitosans on enteropathogens in meat broth”, Journal of Chitin and Chitosan, 2001, 6:22–25.
[22]. HK. No et al., “Applications of chitosan for improvement of quality and shelf life of foods: A review”, Journal of Food Science, 2007, 72:87–100.